Figure 1. In vivo electrophysiological recordings from Cx26^loxP/loxP and Cx26^Sox10Cre mice.

(a) Representative recordings of auditory brainstem responses (ABR) evoked by 14 kHz tone burst stimuli in a Cx26^loxP/loxP mouse (P32, blue line) and a Cx26^Sox10Cre mouse (P39, red line). Note that waves II, IV and V were detected down to 30 dB SPL in the Cx26^loxP/loxP mouse, whereas no evoked responses were identified for intensities ≤90 dB SPL in the Cx26^Sox10Cre mouse. (b) ABR audiograms for tone bursts at 8, 14, 20, 26, 32 kHz and for click stimuli obtained from Cx26^loxP/loxP mice (blue line, n = 12) and Cx26^Sox10Cre mice (red line, n = 12) aged between P29 and P64; error bars represent standard deviation. Note that click responses are plotted at an arbitrary point on the frequency axis (the position does not reflect the frequency content of click stimuli). (c) Representative recordings of endocochlear potential (EP) obtained from a Cx26^loxP/loxP mouse (blue line) and a Cx26^Sox10Cre (red line) mouse, aged P38 and P41 respectively. Standards for reliable recording were: 1) stable EP during minimum 10 seconds and 2) a maximum difference of ±2 mV between starting and final baseline potentials.