Figure 5. Recovery of Cx26 expression in Cx26^Sox10Cre organotypic cultures transduced with BAAVCx26CFP.

Top, immunoreactivity to Cx26 antibodies in a representative Cx26^loxP/loxP culture. Middle, lack of Cx26 immunoreactivity in a representative Cx26^Sox10Cre culture. Bottom, immunoreactivity to GFP antibodies, which also recognize CFP, in a representative Cx26^Sox10Cre culture transduced with BAAVCx26CFP. In all panels, actin filaments were stained with Texas red conjugated phalloidin (red). Scale bar, 10 µm.