Figure 3. Over-expression of FGF-218kDa, FGF-8b, FGF-15 or FGF-17a does not influence differentiation of DA neurons in vitro.
(A–D) Comparative evaluation of E11.5 derived VM cultures from wild-type and FGF-2 deficient mice revealed similar numbers of neurons (ß-tubulin III-ir, A) and DA neurons (TH-ir, B), quantified either by cell-ELISA (C) or immuncytochemistry (D). (E–N) The transient over-expression of FGF-218kDa (G,K), FGF-8b (H;L), FGF-15 (I,M) or FGF-17a (J,N), did not significantly increase the yield of TH-ir cells (K-N) neither in wild-type (E) nor in FGF-2 deficient VM cells (F).