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. 2011 Aug 18;6(8):e23501. doi: 10.1371/journal.pone.0023501

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The node a is X-terminated to the left. The non-reciprocal linkage between nodes b and c is removed because the terminal base (lower case “a” in the sequence) of node c is low quality. Node e is F-terminated to the right. The resultant U-U contig is the union of nodes b and d: CTGCTGCT.