Figure 5. Differences between E. coli meraculous and reference sequence identify transposon insertion.

Bottom line shows portion of the Genbank reference genome for E. coli str. K-12 substr. MG1655 produced by Sanger sequencing and directed finishing strain [27]. Top shows alignment of the de novo meraculus contigs to reference sequence. Solid lines agree perfectly. Angled dashed lines represent unaligned meraculous contig-ends that correspond to the beginning and end of a transposable element. All short-read data supports the meraculous sequence, indicating either insertion of the transposon in the Illumina-sequenced lineage, or an error in the MG1655 reference.