Leptin:	Forward: 5′-CACCAAAACCCTCATCAAGACA-3′
	Reverse: 5′-TAGAGAAGGCCAGCACGTGAA-3′
	Conditions: 95°C 45 seconds; 58°C 45 seconds; 72°C 45 seconds, magnesium chloride 3.0 mM, 38 cycles.
ObRb:	Forward: 5′-CCAGAAACGTTTGAGCATCT-3′
	Reverse: 5′-CAAAAGCACACCACTCTCTC-3′
	Conditions: 94°C 60 seconds; 58°C 60 seconds; 72°C 60 seconds, magnesium chloride 5.0 mM, 35 cycles.