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. 2011 Aug 1;108(33):13534–13539. doi: 10.1073/pnas.1109263108

Fig. 3.

Fig. 3.

Nucleoids are frequently distributed in clusters. (A) Ratio of the number of DNA antibody-labeled nucleoids that were detected with STED microscopy versus those detected by confocal microscopy. The numbers denote the quantity of nucleoids detected by STED microscopy in a confocally assigned nucleoid. (B) Cells labeled with an antiserum against TFAM analyzed as in A. (C) Confocal imaging of BrdU-stained nucleoids in human fibroblasts after 60 min of BrdU incubation. (Scale bar, 2 μm.) (D) STED micrograph of the same section as in C. (Scale bar, 2 μm.) (E) Sizes of nucleoids in human fibroblasts labeled with BrdU antibodies and imaged by confocal and STED microscopy. (F) Cells treated for 60 min with BrdU and labeled with an antiserum against BrdU analyzed as in A. Error bars indicate SEM.