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. 2011 Jun 15;108(33):E472-E479. doi: 10.1073/pnas.1100236108

Fig. 4.

Fig. 4.

Activation of Arp2/3 complex by deletions of VCA–VCA. (A) Cartoon representation of the VCA–VCA materials. VCA is from human WASP. (B) Actin polymerization rates found in Arp2/3 dependent actin polymerization assays with the indicated concentrations of the VCA materials described in A. (C) Actin polymerization rates found in Arp2/3 dependent actin polymerization assays with VCA dimers with different length N-terminal linkers. Horizontal dashed line is the activity of unstimulated Arp2/3. Activity assays were performed with 10 nM Arp2/3, 4 μM Actin (5% pyrene labeled) in KMEI150, and are the average of three to six repeats. Error bars are the 1σ standard error on the mean, and in many cases are smaller than the symbol. See also Fig. S4 in SI Appendix for VCA dimer sequence details, measurements of the affinity of the materials in A for Arp2/3 and for titrations of the N-terminal linker variants.