Fig. 2.

CXCR5^loICOS^lo cells localize exclusively outside GCs. (A) Localization of IL-7R⁺ cells in tonsils was analyzed by immunohistochemistry using a frozen tonsil section. FM, follicular mantle. (Magnification: 4×.) (B and C) Localization of CXCR5^loICOS^lo CD4⁺ T cells was analyzed by the coexpression of CD4 (red), CD45RO (green), and IL-7R (blue) by immunofluorescence microscopy (10×). Triple positive cells illustrated by the white color include CXCR5^loICOS^lo CD4⁺ T cells. A higher magnification view (40×) is shown in C (analyzed by confocal microscopy).