Fig. 3.
Tonsillar Th populations differentially help B-cell subsets. (A–C) Ig production by B-cell subsets cocultured with tonsillar Th populations. (A) GC-B cells (n = 11 except CXCR5loICOShi, which is n = 3), (B) naïve B cells (n = 4), and (C) memory B cells (n = 6 except CXCR5loICOShi, which is n = 3) were cocultured with each Th population in the presence of SEB for 8 d. One-way ANOVA Bonferonni multiple comparison test. ***P < 0.001, **P < 0.01, and *P < 0.05. (D and E) Recovery of GC-B cells cocultured with Th populations. GC-B cells cultured for 8 d with each Th population were stained with CD3 and CD4 mAbs. Surface IgG and IgA expression by B cells cultured with CXCR5hiICOShi GC-Tfh cells is shown in Right (a representative from four experiments). Absolute number of viable GC-B cells per well (day 8) are shown in E (n = 5). One-way ANOVA Bonferonni multiple comparison test. (F) Recovery of T cells cocultured with GC-B cells. Absolute number of viable T cells per well (day 8; n = 5). One-way ANOVA Bonferonni multiple comparison test. (G) Proliferation and CD38 expression of CFSE-labeled naïve B cells cultured for 8 d with each Th population (a representative from three experiments). (H) Recovery of naïve B cells cocultured with Th populations. Absolute number of viable CD38+ plasmablasts per well (day 8; n = 3). One-way ANOVA Bonferonni multiple comparison test. (I) Recovery of T cells cocultured with naïve B cells. Absolute number of viable T cells per well (day 8; n = 3). One-way ANOVA Bonferonni multiple comparison test.