Fig. 8.

CXCR5^loICOS^lo CD4⁺ T cells in adult tonsils. The four Th populations in pediatric tonsil samples, adult tonsil samples, and human spleen samples. The same number of T-enriched cells (after removing B cells with magnetic beads) was stained and analyzed in parallel in identical conditions. The four Th populations were determined based on the analysis of pediatric tonsil samples, and the same gates were applied for the adult and spleen samples. (A) A representative of at least two experiments gated to CD3⁺CD4⁺ T cells. (B) The frequency of each Th population in adult tonsils (n = 3) and spleen (n = 2). One-way ANOVA Bonferonni multiple comparison test for adult tonsil samples. *P < 0.05.