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Topologies of the pSTB plasmid in single copy state in G1 arrested or nocodazole-treated cells. (A–D) The single-copy pSTB and CEN4 circle were generated in G1 by recombination (see Fig. 2). Cells were released from G1 in galactose with the addition of nocodazole (Noc) or without nocodazole as the control (Con). Topology analyses were performed in G1 arrested cells (lane 2) or G2/M cells (Con, lane 1; Noc, lane 3).
