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Individual contributions of STB and CEN to the topology of a reporter plasmid harboring both loci. (A and B) The indicated strains harboring the pSTB-CEN4’ reporter plasmid (Fig. 1) were arrested in G1 at 26 °C, conditioned with galactose, and released in galactose at 26 °C or 37 °C. Plasmid topologies were assayed at 2 h after release.
