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Splicing of intron 2 is required for normal H3K36 methylation. (Upper) Maps of the integrated WT CMV β-globin gene and the intron1 and intron 2 3′ splice site deletions. Relative ChIP signals are shown for (A) pol II relative to the hyg 782 amplicon as in Fig. 1A, (B) U5 100 K normalized to pol II, and (C) H3K36me3 normalized to H3 as in Fig. 1. Means of n PCRs and SEMs are shown.
