Fig. 3.

Induction of apoptosis in naive CD8⁺ T cells by scK^d.IGRP tetramers. (A) Splenocytes from 6- to 8-wk-old 8.3 TCR-transgenic NOD mice were incubated at 37 °C for 3 h with 25 nM scK^d.IGRP tetramers (red histogram) or 25 nM scK^d.TUM tetramers (blue) or left untreated (filled gray), and then stained with anti-CD8 and anti-CD62L and analyzed by flow cytometry. Samples were gated on CD8⁺ cells. (B) As in A, except that treated and untreated splenocytes were stained with anti-CD8, Annexin V, and 7-AAD and analyzed by flow cytometry. Samples were gated on CD8⁺ 7-AAD⁻ cells. (C) As in B, except that splenocytes from 6- to 8-wk-old AI4 TCR-transgenic NOD mice were used. In A–C, numbers denote the percentage of cells present in the indicated quadrants of the dot plots.