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. 2011 Aug 8;108(33):13734–13739. doi: 10.1073/pnas.1105143108

Table 3.

HHV-6 primers/probe sets used in PCR reactions

Primers/probe sets
Amplicon size
Nucleotide sequence 5′→3′ DNA cDNA PCR cycle conditions
U67 HHV-6 Ext-F- 5′-GCGTTTTCAGTGTGTAGTTCGGCAG-3′ 524 92 °C:3 min, 92 °C:30 s, 55 °C:30 s, 72 °C:30 s (35 cycles), 72 °C:7 min, 4 °C
Ext-R- 5′-TGGCCGCATTCGTACAGATACGGAGG-3′
Int-F- 5′-GCTAGAACGTATTTGCTGCAGAACG-3′ 258 92 °C:3 min, 92 °C:30 s, 55 °C:30 s, 72 °C:30 s (35 cycles), 72 °C:7 min, 4 °C
Int-R- 5′-ATCCGAAACAACTGTCTGACTGGCA-3′
U12 HHV-6 Ext-F-5′-CACTGTCATTGAGCTGTCCAA-3′ 327 238 94 °C:5 min, 94 °C:30 s, 57 °C:30 s, 72 °C:30 s (35 cycles), 72 °C:7min, 4 °C
Ext-R-5′-ACCACATGAGCACAAAATCG-3′
Int-F-5′-CACTGTCATTGAGCTGTCCAA-3′ 202 113 94 °C:5 min, 94 °C:30 s, 57 °C:30 s, 72 °C:30 s (35 cycles), 72 °C:7min, 4 °C
Int-R-5′-TGAGCGTGATTCCGGTAACT-3′
U90 HHV-6A F-5′-CATGAAGATGATGACAATAAAATG-3′ 278 94 °C:3 min, 94 °C:20 s, 65 °C:50 s (45 cycles), 4 °C
R-5′-TGGAACCATCTTGTTCTGTCC-3′
FAM-CCGCCCAGATCTGTCACTGAGGCG-TAMRA
U90 HHV-6B F-5′-GAGACCGGGTCTGGACAACA-3′ 145 94 °C:3 min, 94 °C:20 s, 65 °C:50 s (45 cycles), 4 °C
R-5′-GAGTTGCTGAGTTGGTAAAGG-3′
FAM-CTCCAAGTGTACCGAAACGCTTCCTGG-TAMRA
β-Actin F-5′-CACACTGTGCCCATCTACGA-3′ 107 94 °C:3 min, 94 °C:20 s, 65 °C:50 s (45 cycles), 4 °C
R-5′-CTCAGTGAGGATCTTCATGAGGTAGT-3′
FAM-ATGCCCTCCCCCATGCCATCCTGCGT-TAMRA
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