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. 2011 Aug 3;108(33):13694–13699. doi: 10.1073/pnas.1107594108

Fig. 6.

Fig. 6.

Immune responsiveness is preferentially maintained within old Vb10+ gB-8p precursors. (A) Different aged mice were infected with 5,000 CFU LM-gB and given BrdU in their drinking water. At 3 d postinfection, gB-8p cells were isolated and the percentage of BrdU+ cells within the Vβ10+ and Vβ10− fractions were evaluated. (B) LM-gB infected mice were pulsed with 0.8 mg BrdU (i.p.) on day 6 and the percentage of BrdU+ cells was analyzed in splenic Vβ10+ and Vβ10− gB-8p cells on day 7 postinfection. (C) Polyfunctional CD8+ T cells or those that are capable of simultaneously producing IFN-γ, TNF-α, and Granzyme B following gB-8p peptide stimulation were calculated at 7 d postinfection. (D) The MFI of Granzyme B produced individually by Vβ10+ and Vβ10− gB-8p-specific CD8+ T cells is also shown. Data shown in A were pooled from two separate experiments, with n = 3 mice per group per experiment. Data shown in B to D are representative of at least two separate experiments with n = 5–8 mice per group. Results depict mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.