Fig. 2.

Localization of MamA, its N-terminally-truncated versions and mutants fused to GFP in a M. magneticum AMB-1 ΔmamA strain. Shown is a representative cell for each strain. For each strain, 300 cells were analyzed in independent experiments (see Fig. S4 for more cell images). MamA displays a linear localization pattern reminiscent of the magnetosome chain reported before (9). MamAΔ26 and MamAΔ41 share a diffuse cellular localization pattern in all analyzed cells, while the fluorescence intensity of MamAΔ26 is significantly lower. Note that the image of the MamAΔ26 cell was electronically enhanced (green frame) to show fluorescence. MamA R50E displays a largely diffuse localization pattern (∼80% of all cells) but occasionally also a linear pattern, whereas MamA D159K displays a linear localization pattern alone.