Table 3.
Effects of TNF during in vitro maturation on meiotic maturation and subsequent embryonic development of porcine oocytes following IVF/IVC*.
TNF concentration |
Percentage of oocytes reaching to MII (n) |
Embryonic cleavage (n) |
Blastocyst development (n) |
Blastocyst/cleaved § | Blastocyst cell number (n) |
---|---|---|---|---|---|
0 ng/ml | 66.1 ± 6.1% (62) | 57.8 ± 2.9% (288) | 23.0 ± 2.7% (288) | 39.4 ± 4.0% | 45.0 ± 2.3 (65) |
0.1 ng/ml | 62.5 ± 6.5% (58) | 64.2 ± 3.0% (249) | 25.8 ± 2.8% (249) | 39.7 ± 3.9% | 43.1 ± 2.8 (63) |
1 ng/ml | 50.0 ± 7.3% (48) | 63.2 ± 2.7% (279) | 26.1 ± 3.7% (279) | 38.2 ± 4.8% | 55.3 ± 2.8 (72) |
Data are reported as mean ± SEM. No significant differences (P > 0.05) were found in percentage of oocytes reaching to MII and embryonic development in all treatment groups.
Percentage of blastocysts is calculated from the cleaved embryos.