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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Aging Cell. 2011 May 25;10(5):780–788. doi: 10.1111/j.1474-9726.2011.00717.x

FIG. 2.

FIG. 2

Effects of age on CREB activation by PTH peptides in hMSCs. (A) Western immunoblot shows that after 6 hours treatment, hPTH1-31C, hPTH1–34, and hPTH1–84 (10 nM) increased phospho-CREB levels, but not CREB, in hMSCs (representative immunoblot with hMSCs from a 34-year-old female subject). (B) Quantitative data from Western immunoblot are presented as fold-difference of p-CREB with PTH treatment compared with vehicle control after normalization to each β-actin internal control. (C) The effect of PTH1–34 on p-CREB was inversely correlated with subject age (Pearson r=−0.80, p=0.0294, n=7). INSET: The mean effect of PTH1–34 on p-CREB was significantly higher in the young group (<50 years, n=4) than in the group of subjects older (>55 years, n=3) (*p<0.05, Mann-Whitney test).