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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Aging Cell. 2011 May 25;10(5):780–788. doi: 10.1111/j.1474-9726.2011.00717.x

FIG. 4.

FIG. 4

Effects of age on stimulation of osteoblastogenesis by PTH peptides in hMSCs. (A) Electrophoretogram of RT-PCR products shows upregulation of IGF-I, IGF-II and ALP genes in hMSCs (34F), after 3 days treatment with PTH peptides (10 nM) in osteogenic medium. GAPDH was used as an internal control. (B) The PTH peptides (10 nM) produced significant increases in ALP activity at 1 week (*p<0.05, **p<0.01, ANOVA). (C) The PTH peptides (10 nM) produced significant increases in mineralization at 3 weeks (*p<0.05, ANOVA). (D) PTH1–34 (10 nM) stimulated ALP activity, compared with control, set at 1.0, in cells from young subjects (<50-year-old, n=5) 3.7-fold more than in cells from older subjects (>55-year-old, n=7) (p=0.042, Mann-Whitney test).