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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2011 Apr 9;155(1):136–142. doi: 10.1016/j.cbpc.2011.04.002

Figure 2. Maintenance and expansion of mouse cells from cryopreserved tissue in selective media.

Figure 2

Thawed and dissociated mouse lung cells were passed twice in DMEM-F medium and plated at 2 × 104 cells/35mm dishes in DMEM-F, SAEC or EGM-2 media. At 80–90% confluence, cells were counted and replated at the same starting density. Panels A and B are linear and 3rd order polynomial plots of the same data sets. Day 0 represents the day in which disaggregated cells were plated on culture dishes.