Figure 4.

G protein expression in whole brain homogenates from Gα_o transgenic mice. Membranes from whole brain of wild-type (wt), Gα_o +/− (+/−), and Gα_o −/− (−/−) mice were separated by SDS-PAGE, transferred to nitrocellulose membranes, and probed for the expression of (a) Gα_o, (b) Gα_z, (c) Gα_i1, (d) Gα_i2, (e) Gβ₁₋₄, or (f) Gγ₂ using selective antibodies (see Materials and Methods section); membranes were also probed for tubulin as a loading control. G protein expression was quantified in Image J by normalizing G protein band intensity to tubulin band intensity, and data are plotted as a ratio of wt expression. Data represent the mean±SEM (n=3). Symbols indicate a statistical difference vs wt (^*p<0.05, ^**p<0.01, ^***p<0.001) or +/− (⁺p<0.05, ⁺⁺p<0.01, ⁺⁺⁺p<0.001) by Bonferroni's post-test.