Fig. 3.

Restoration of XKS1 activity. A 100 μL of purified XKS1 (specific activity: 0.1 U/mg; protein concentration: 2 mg/mL) were incubated with 100 μL of E. coli BL21 DE3 crude extract (protein concentration: 40 mg/mL) for 400 min at 25 °C (●). The same experiment was performed using 100 μL of S. cerevisiae CEN.PK-113-5D crude extract (protein concentration: 40 mg/mL) for 400 min at 25 °C (■). The specific activity of XKS1 was determined at defined points using the standard XKS1 activity assay (see Materials and Methods).