Table 3.
Distinct peptides identified by LC-MSMS analysis of S. cerevisiae Xylulokinase XKS1 (NCBI Nr. 6321633). The two conserved catalytic aspartate residues D28 and D299 are indicated in bold. The sequence coverage was 92%.
| Peptide sequence | Experimental parent mass (Da) | Mass error (Da) | Start residue |
|---|---|---|---|
| (M)SLDSYYLGFD28LSTQQLK(C) | 1977.98 | 0.0015 | 18 |
| (K)CLAINQDLK(I) | 1074.561 | −0.0003 | 35 |
| (C)LAINQDLKIV(H) | 1126.683 | 0.0003 | 36 |
| CK)IVHSETVEFEK(D) | 1317.669 | −0.0002 | 44 |
| CK)DLPHYHTKKGVYIHG(D) | 1764.918 | 0.0009 | 55 |
| (K)KGVYIHGDTIECPVAMWLEALDLVLSK(Y) | 3057.579 | 0.002 | 63 |
| (K)VMAVSGSCQQHGSVYWSSQAESLLEQLNK(K) | 3223.515 | 0 | 100 |
| (E)KDLLHYVSSVAFARQTAPNWQDHSTAKQCQE(F) | 3615.74 | −0.0108 | 132 |
| (A)KQCQEFEECIGGPEKM(A) | 1969.845 | 0.0009 | 158 |
| (K)MAQLTGSRAHFRFTGPQILK(T) | 2259.218 | 0.0003 | 173 |
| (K)IAQLEPEAYEK(T) | 1290.658 | −0.0007 | 193 |
| (E)KTKTISLVSNFLTSILVGHLVE(L) | 2399.391 | 0.0034 | 203 |
| (K)TISLVSNFLTSILVGHLVELEEADACGMNLYDIRERK(F) | 4206.157 | 0.004 | 206 |
| (K)FSDELLHLIDSSSKDK(T) | 1833.923 | 0.0002 | 243 |
| (K)LMRAPMK(N) | 846.469 | −0.0003 | 264 |
| (K)NLIAGTICK(Y) | 989.545 | 0.0001 | 271 |
| (G)TICKYFIEKYGFNT(N) | 1783.872 | 0.002 | 276 |
| (K)VSPMTGD299NLATICSLPLRK(N) | 2073.083 | −0.0001 | 293 |
| (K)NDVLVSLGTSTTVLLVTDK(Y) | 1975.096 | 0 | 312 |
| (L)LVTDKYHPSPN(Y) | 1270.643 | −0.0001 | 326 |
| (N)YHLFIHPT(L) | 1027.536 | −0.0005 | 337 |
| (R)IRDELNKEREN(N) | 1415.724 | −0.0002 | 365 |
| (K)TNDWTLFNQAVLDDSESSENELGVYFPLGEIVPSVK(A) | 4012.934 | 0.0014 | 380 |
| (E)IWSVKAINKRVTFNPKTGMIERE(V) | 2739.57 | 0.0015 | 410 |
| (E)VAKFKDKRHDAKNIVE(S) | 1898.061 | 0.0003 | 434 |
| (H)DAKNIVESQALSCRVRISPLLS(D) | 2456.329 | 0.0023 | 443 |
| (R)ISPLLSDSNASSQQRLNEDTIVKFDYDESPLR(D) | 3637.798 | −0.002 | 459 |
| (R)DYLKKRPERTFFVGGASKN(D) | 2199.131 | −0.0013 | 491 |
| (D)AIVKKFAQVIGATKGNFRLE(T) | 2190.276 | 0.0005 | 511 |
| (K)GNFRLETPNSCALGGCYK(A) | 2043.938 | −0.001 | 525 |
| (K)AMWSLLYDSNK(I) | 1327.635 | −0.0006 | 543 |
| (I)AWFDKFLNDNFPWHV(M) | 1945.959 | −0.0003 | 555 |
| (K)FLNDNFPWHVMESISDVDNENWDRYNSKIVPLSELEK(T) | 4480.119 | 0.0115 | 561 |