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The growth of CD133+ or CD133− selected cells from cultures of primary benign epithelia or BPH-1 cell line, in Matrigel. The 3,000 (primary) or 4,000 (BPH-1) cells/mL were plated (as single cells) and grown for 10 days in 4% (v/v) Matrigel, containing stem cell media (SCM) + 2% FCS. Scale bar = 50 μm.
