Fig. 3.

Growth of CD133⁺ cells and stromal aggregates in Matrigel. CD133⁺ or CD133⁻ cells were isolated from prostate epithelial cultures. Fifty cells per well were aggregated with or without 100 stromal cells/well (in nonadherent 96-well plates), in a volume of 100 μL stem cell media (SCM) for 1 week. Aggregates were then grown in 4% (v/v) Matrigel for a further 2 weeks, after which times images were collected. Epithelial cells grown with STOs were pre-stained with red cell tracker dye to locate them within aggregates. Fluorescent images were collected at equivalent exposure settings. Images were taken at 4× magnification. Scale bars = 50 μm.