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Protein sequences of the Hha variants from screening altered biofilm dispersal and biofilm formation.
| Hha variants | Replacements |
|---|---|
| Hha dispersal variants | |
| Hha13D6 | D22V, L40R, V42I, D48A |
| Hha20D7 | D48G, R50C |
| Hha saturation mutagenesis variant | |
| HhaSM2C1 | D22M, L40R, V42I, D48A |
| Hha biofilm variants | |
| Hha1D8 | L6I, E34V, N38E, K58E, K62X |
| Hha1D9 | E53X |
| Hha12H6 | V67E |
| Hha13B3 | Q19R, N57D |
| Hha24E9 | K62X |
| Hha dispersal‐biofilm combined variant | |
| Hha13D6‐24E9 | D22V, L40R, V42I, D48A, K62X |
X indicates termination.
