Table 3.
Escherichia coli K‐12 strains and plasmids used in this study.
| Strains and plasmids | Genotype/relevant characteristics | Source |
|---|---|---|
| Strains | ||
| AG1 | recA1 endA1 gyrA96 thi‐1 hsdR17(rK‐mK+) supE44 relA1 | Kitagawa et al. (2005) |
| BW25113 | lacIqrrnBT14ΔlacZWJ16hsdR514 ΔaraBADAH33ΔrhaBADLD78 | Baba et al. (2006) |
| BW25113 hha | BW25113 ΔhhaΩ KmR | Baba et al. (2006) |
| BW25113 tnaA | BW25113 ΔtnaAΩ KmR | Baba et al. (2006) |
| BW25113 clpB | BW25113 ΔclpBΩ KmR | Baba et al. (2006) |
| BW25113 hslU | BW25113 ΔhslUΩ KmR | Baba et al. (2006) |
| BW25113 hslV | BW25113 ΔhslVΩ KmR | Baba et al. (2006) |
| BW25113 lon | BW25113 ΔlonΩ KmR | Baba et al. (2006) |
| BW25113 prlC | BW25113 ΔprlCΩ KmR | Baba et al. (2006) |
| BW25113 ycjF | BW25113 ΔycjFΩ KmR | Baba et al. (2006) |
| BW25113 gadA | BW25113 ΔgadAΩ KmR | Baba et al. (2006) |
| BW25113 gadE | BW25113 ΔgadEΩ KmR | Baba et al. (2006) |
| BW25113 gadX | BW25113 ΔgadXΩ KmR | Baba et al. (2006) |
| BW25113 ibpA | BW25113 ΔibpAΩ KmR | Baba et al. (2006) |
| BW25113 ibpB | BW25113 ΔibpBΩ KmR | Baba et al. (2006) |
| BW25113 gadW | BW25113 ΔgadWΩ KmR | Baba et al. (2006) |
| BW25113 glpT | BW25113 ΔglpTΩ KmR | Baba et al. (2006) |
| BW25113 phnF | BW25113 ΔphnFΩ KmR | Baba et al. (2006) |
| BW25113 hha tnaA | BW25113 ΔhhaΔtnaAΩ KmR | This study |
| BW25113 hha clpB | BW25113 ΔhhaΔclpBΩ KmR | This study |
| BW25113 hha hslU | BW25113 ΔhhaΔhslUΩ KmR | This study |
| BW25113 hha hslV | BW25113 ΔhhaΔhslVΩ KmR | This study |
| BW25113 hha lon | BW25113 ΔhhaΔlonΩ KmR | This study |
| BW25113 hha prlC | BW25113 ΔhhaΔprlCΩ KmR | This study |
| BW25113 hha ycjF | BW25113 ΔhhaΔycjFΩ KmR | This study |
| BW25113 hha gadA | BW25113 ΔhhaΔgadAΩ KmR | This study |
| BW25113 hha gadE | BW25113 ΔhhaΔgadEΩ KmR | This study |
| BW25113 hha gadX | BW25113 ΔhhaΔgadXΩ KmR | This study |
| BW25113 hha ibpA | BW25113 ΔhhaΔibpAΩ KmR | This study |
| BW25113 hha ibpB | BW25113 ΔhhaΔibpBΩ KmR | This study |
| Plasmids | ||
| pCA24N | CmR; lacIq, pCA24N | Kitagawa et al. (2005) |
| pCA24N‐hha | CmR; lacIq, pCA24N PT5‐lac::hha+ | Kitagawa et al. (2005) |
| pCA24N‐yddV | CmR; lacIq, pCA24N PT5‐lac::yddV+ | Kitagawa et al. (2005) |
| pCM18 | EmR, GFP+ | Hansen et al. (2001) |
| pCP20 | ApR and CmR plasmid with temperature‐sensitive replication and thermal induction of FLP synthesis | Cherepanov and Wackernagel (1995) |
KmR, CmR, EmR and ApR are kanamycin, chloramphenicol, erythromycin and ampicillin resistance respectively.