Figure 4. Lack of gross morphological abnormalities in L1CD mutant mice. A, F, L: WT; B, G, M: L1^Y1176A; C, H, N: L1¹¹⁸⁰; D. I, O: L1¹¹⁵²; E, J, P: L1^KO.

(A-E) No hydrocephalus in L1CD mutant mice. Coronal section from brains showed that there are no enlarged ventricles in L1CD mice, although L1^KO mice have enlarged ventricles. n = 3. Scale bar, 200 μm

(F-J) Normal morphology of cerebellar lobe in mid-sagittal sections of brains. The cerebellar lobule shows normal morphology in L1CD mutant mice. n=3. Scale bar, 1 mm.

(K) Morphometric analysis of cerebellum at the midline of brains. There is no significant difference in the size of the cerebellum of L1CD mutant mice, although the L1^KO mice cerebella are significantly smaller. n = 3. *p < 0.05.

(L-P) Cresyl violet staining for hippocampus region. Formation of hippocampus is normal in L1CD mutant mice compared with L1^WT mice, although L1^KO mice have a smaller hippocampus. Scale bar = 500 μm