Figure 6.

Genetically encoded PKMζ inhibitory pseudosubstrate suppresses synaptic increase of PSD-95 in vivo after eye opening. A, Nucleotide and amino acid sequences of the 2A cleavage motif followed by either the scrambled peptide or the PKMζ pseudosubstrate peptide in the expression plasmids of TagRFP-T. B, Endogenous dendritic PSD-95 puncta are decreased in neurons expressing the pseudosubstrate but not in neurons expressing scrambled peptide or untransfected neurons (data not shown). C, Visual cortical neurons electroporated at E15.5 with constructs coding for either the pseudosubstrate-RFP or the scrambled-peptide-RFP plus PSD-95-GFP. Insets depict pseudosubstrate-RFP or scrambled-peptide-RFP. The diagram (top) illustrates the sequence of in vivo experiments. D, Visual cortical neurons expressing PKMζ inhibitory pseudosubstrate showed a significant reduction in PSD-95 puncta intensity compared with neurons expressing the scrambled peptide. Scale bars: 10 μm.