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p41-Arc induces senescence in human diploid fibroblasts. (A) Growth of HDF expressing p41-Arc. HDF at passage 15 were retrovirally transduced with p41 or empty vector (pBabe). 1 × 104 of puromycin-resistant cell pools were grown for the indicated periods and then cell numbers were counted at each passage. (B) Proportion of SA-β-gal stained HDF. SA-β-galactosidase activity was assayed at each passage. (C) Expression of p41-Arc in HDF.
