Figure 2.

Effect of 5-aza-Cd on the expression of BART miRNAs and BART promoter. (A) CpG methylation status of the BART promoter was analyzed by pyrosequencing in Mutu I, Namalwa, Mutu III, and Akata-EBV cell lines which were treated with 5-aza-Cd for 72 h. (B) Northern blot was performed for EBV miRNAs using total RNA isolated from the cells treated with the indicating concentrations of 5-aza-Cd for 72 h. To facilitate analysis of the changed expression levels of BART miRNAs following 5-aza-Cd treatment, different optimal exposure time was used for each cell line and for each individual BART miRNA. Loading amount of each RNA sample was monitored by reprobing the blot with a specific probe to U6 snRNA. The expression of human miR-16 was assessed as a reference.