Table 2.
Effect of diclofenac on antibiotic susceptibility of COL, SH1000 and DcRS mutant derivatives
| MICa (μg/ml) | ||||||
|---|---|---|---|---|---|---|
| Antibiotic | Strain | Control | Dcb (32 μg/ml) | FI/FDc | Dc (64 μg/ml) | FI/FD |
| Ciprofloxacin | SH1000 | 0.5 | 0.25 | -2 | 0.125 | -4 |
| SC1-SC3d | 0.5 | 0.25 | -2 | 0.125 | -4 | |
| COL | 0.5 | 0.25 | -2 | 0.25 | -2 | |
| SC4-SC6d | 0.5 | 0.5 | 0 | 0.25 | -2 | |
| BB255 | 0.25 | 0.125 | -2 | 0.125 | -2 | |
| WGB8287 | 0.5 | 0.125 | -4 | 0.125 | -4 | |
| SA1199B | 8 | 4 | -2 | 4 | -2 | |
| WBG9312 | 32 | 4 | -8 | 4 | -8 | |
| Norfloxacin | Alle | 0.125 | 0.0625 | -2 | 0.0625 | -2 |
| Ofloxacin | SA1199B | 2 | 1 | -2 | 0.5 | -4 |
| WBG9312 | 8 | 4 | -2 | 4 | -2 | |
| Oxacillin | SH1000 | 0.25 | 0.25 | 0 | 0.5 | 2 |
| SC1-3 | 0.25 | 0.5 | 2 | 0.5 | 2 | |
| COL | >256 | >256 | ND | >256 | ND | |
| SC4-6 | >256 | >256 | ND | >256 | ND | |
| BB255 | 0.25 | 0.25 | 0 | 0.25 | 0 | |
| WGB8287 | 32 | 64 | 2 | 128 | 4 | |
| SA1199B | 0.13 | 0.25 | 2 | 0.5 | 4 | |
| WBG9312 | 2 | 8 | 4 | 16 | 8 | |
a Minimum inhibitory concentration (MIC).
b Diclofenac (Dc).
c Fold increase (FI) or fold decrease (FD) in MIC and in the presence of Dcl.
d DcRS mutant derivative isolates of SH1000 (SC1 through SC3) all had the same MICs; those of COL (SC4 through SC6) also all had the same MICs.
e All (all strains in the study expressed the same MIC: SH1000, COL, SC1-SC6, BB255, WGB8287, SA1199B, and WBG9312).