Figure 1.

Effects of Sal B on growth of human oral squamous cell carcinoma cells CAL27, SCC4 and oral precancerous cells Leuk1. Cells (1 × 10⁴) were treated with increasing concentrations of Sal B (50, 100,200 μg/mL) for 72 h. Viable cells were measured by MTT assay and expressed as a percentage of control. All values are means of three independent experiments ± SD (bars). Time-dependent growth inhibition was seen in CAL27 cells. The SCC4 cells seemed to be stimulated after 24 h, but moderate inhibited at higher concentration after 48 h and inhibited after 72 h. No growth inhibition was also seen in Leuk1 cells. The significance of results obtained from the control and treated groups was analyzed using the paired Student's t-test. Means and standard deviations were calculated. P < 0.05 was regarded as statistically significant.