. Author manuscript; available in PMC: 2011 Aug 19.

Published in final edited form as: Biochemistry. 2009 Jan 20;48(2):346–356. doi: 10.1021/bi8009407

Table 1.

Crystallization Conditions for GlpK Mutant Enzymes

Enzyme	Crystallization conditions	Glycerol added	Space Group
His232Arg + glycerol	0.05M KH₂PO₄, 17% (w/v) PEG 8000	10% (Co-crystallized)	P2₁2₁2
His232Arg + ethylene glycol	0.05M KH₂PO₄, 17% (w/v) PEG 8000	0% Soaked with 23% ethylene glycol	P2₁
His232Arg-B + glycerol	2.0M (NH₄)₂SO₄, 5% (v/v) isopropanol	0% 15% present during purification (Co-crystallized)	P2₁2₁2
His232Glu + glycerol	0.1M HEPES pH 6.5, 10mM ZnSO₄, 25% (w/v) PEG 550 MME	10% (Co-crystallized)	P2₁2₁2
His232Glu + ethylene glycol	0.05M KH₂PO₄, 20% (w/v) PEG 8000	0% Soaked with 25% ethylene glycol	P2₁
His232Ala + glycerol^a	29% PEG 400, 0.1 M sodium acetate pH 4.5, 0.1 M calcium acetate	10% (Co-crystallized)	P2₁2₁2