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. Author manuscript; available in PMC: 2011 Aug 19.
Published in final edited form as: J Pineal Res. 2010 May 27;49(1):60–68. doi: 10.1111/j.1600-079X.2010.00767.x

Fig. 4.

Fig. 4

Effect of melatonin on Per2, Clock and Bmal1 mRNA and protein levels in human PCa cells. A) Effect of melatonin on Per2, Clock, and Bmal1 mRNA levels: The relative expression levels of Per2, Clock, and Bmal1 transcripts in PCa cell lines treated with melatonin (1mM or 2mM; 24 hours) were determined by qRT-PCR. The data (relative density normalized to GAPDH) is expressed as mean ± SD of three experiments (*p ≤ 0.01); B) Effect of melatonin on Per2 and Clock protein levels: The protein levels of Clock and Per2 were determined in PCa cells were treated with melatonin (100 μM, 1mM or 2mM; 48 hours) by Western blot analysis. Equal loading was confirmed by reprobing the blot for β-actin. Details of the experiments are given in “Materials and Methods.”