Table 2.
Gene-specific primer sequences, annealing temperatures and MgCl2 concentrations used for real-time RT-PCR
| Gene | EST # | Product length (bp) | Forward primer sequence | Reverse primer sequence | Annealing temp. (°C) | MgCl2 (mm) |
|---|---|---|---|---|---|---|
| GCN5L | K-D4-A01 | 300 | ACTGGCATCTGGGCGTGTG | ATGAAGCGGGCAATCGTGAC | 60·0 | 3 |
| HUB2 | K-D7-D01 | 433 | AGCCCCATGACGCACTGT | GCCTCGGTAAGCTCCAAAAC | 55·0 | 3 |
| GOLS | K-D8-G07 | 244 | AGATGATTTACCTAGACGGAGACA | AAAGAAGCCAGCATTGAAGTAT | 53·4 | 5 |
| RADSAM | L1-2–40 | 596 | CCGGTTCAATCTGGGAGTG | TTAGTGCAAGGCGAACATTTAG | 60·0 | 3 |
| SAMS | K-G4-C06 | 240 | AGGGCCATTGCTCAGGTCTCT | CCAGGTGAAGTCAGGGTCGTC | 60·0 | 1·5 |
| AUR3 | K-G2-H03 | 174 | AACGCATTTTCTTGATTCTCG | ATATACGTGGCAGCTTTGTTCTCG | 60·0 | 3 |
| HSPTF | K-G5-A04 | 167 | CGAGTTGCCCGAGTTTCTTTC | GGCTTTTGCACCATTGGACTATTA | 55·0 | 3 |
| HSPTF90 | K-G4-D02 | 136 | ACTTTGGTGAGGTCCTTGGTAGAG | AGGGAAATTGGGTAGCTGATAAAC | 55·0 | 3 |
| ACTIN | 188 | TCCATCATGAAGTGCGATGT | AACCTCCGATCCAGACACTG | 54·8 | 4·5 |