Figure 4.

AIH decreases apoptosis in the infarct area. (A) A representative immuno-cytochemical stain is shown for samples from each group at 24 h post-reperfusion after LAD ischemia (magnification, x100). The nuclei are stained by DAPI (blue) and the apoptotic cells are displayed using fluorescently modified nucleotide (pink). (B) Quantification of TUNEL positive-staining cells in each of the respective experimental groups. Data are presented as mean cell numbers per square millimeter ± SEM; n = 8 for each group. #, P < 0.01 compared with the normothermia group.