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. 2011 Jul 28;3(4):392–403.

Figure 2.

Figure 2

Characterization of 4T1-HaloTag-ECS cells. A. 4T1-HaloTag-ECS cells are labeled with AF488-HTL (not cell membrane permeable) and then with TMR-HTL (cell membrane permeable). Microscopy images confirmed that the cells express the HaloTag protein both on the surface of and inside the cells. B. Binding of NOTA-HTL-S/M/L to the HaloTag protein was assessed by exposing 4T1-HaloTag-ECS cells to each ligand prior to labeling with the cell membrane impermeable AF488-HTL. Confocal images showed significantly less AF488-HTL signal for NOTA-HTL-S, indicative of its efficient binding to HaloTag, but not for NOTA-HTL-M/L Scale bars: 20 μm.