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. 2011 Jul 28;3(4):392–403.

Figure 3.

Figure 3

Characterization of 4T1-HaloTag cells. A. The cells are labeled with TMR-HTL and examined under a microscope, which confirmed HaloTag protein expression in the cells. B. Cell membrane permeability of NOTA-HTL-S/M/L was assessed by exposing 4T1-HaloTag cells to each ligand prior to labeling with the cell membrane permeable TMR-HTL. Confocal images showed no significant difference in TMR-HTL labeling between control cells (labeled with TMR-HTL only) and cells previously exposed to each NOTA-conjugated HTL, indicating a lack of cell membrane permeability for NOTA-HTL-S/M/L. Scale bars: 20μm.