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. 2011 Aug 19;6(8):e22991. doi: 10.1371/journal.pone.0022991

Figure 3. PTU alters IGF regulation of craniofacial muscle development.

Figure 3

72 hpf Tg(α-actin::EGFP) embryos that were injected with morpholinos against igf1ra (A–D), igf1rb (E–H), or control (globin; I–L) and were raised in control media (A, B, E, F, I, J) or with 0.003% PTU at 12 hpf (C, D, G, H, K, L). Morpholino knockdown of igf1ra or igf1rb in the roy background (in the absence of PTU; A, B, E, F) did not disrupt craniofacial development as compared to control (I, J). Morpholino knockdown of igf1ra in the presence of 0.003% PTU caused developmental delay only in the presence of 0.003% PTU (C, D compared to K, L). Knockdown of igf1rb inhibited jaw formation and caused mild thickening of extraocular muscles in the presence of 0.003% PTU (G, H), but not in control media (E, F). IR, inferior rectus; IO, inferior oblique; AM, anterior mandibulae.