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. 2011 Aug 19;6(8):e22991. doi: 10.1371/journal.pone.0022991

Figure 6. 0.003% PTU Inhibits T4 in thyroid follicles.

Figure 6

Wholemount immunostaining for T4 in 96 hpf embryos (ventral view) raised in control media (A, B) or in media containing 0.003% PTU. PTU was added at 4, 8, 12, 16, 20, or 24 hpf and carried through until the embryos were harvested at 96 hpf. Untreated 96 hpf embryos showed numerous thyroid follicles that contained T4 (A, B). Addition of 0.003% PTU between 4 and 12 hpf suppressed T4 in follicles at 96 hpf (C, E). Addition of 0.003% PTU between 16 and 20 hpf progressively increases T4 staining and treatment at 24 hpf does not affect T4.