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. 2011 Aug 19;6(8):e23387. doi: 10.1371/journal.pone.0023387

Figure 5. Differential regulation of Bmp4 and Bmp7 expression by Lhx2.

Figure 5

(A–P) In situ hybridisation analyses of coronal sections of the optic vesicles in control and mutant (Lhx2-Cre:Lhx2flox/flox) embryos at the indicated developmental stages. A,C,E,H,K,N are in situ hybridisation analyses to detect exon 2 (exon2) in the Lhx2 mRNA to reveal the domain in the optic vesicle where the Lhx2 gene has been inactivated. B,D,F,I,L,O are in situ hybridisation analyses to detect expression of Bmp4. G,J,M,P are in situ hybridisation analyses to detect expression of Bmp7. Bmp4 expression is initiated dorsally in control embryos (B) and in the conditional mutants (D) at ss18–21 (arrows). Bmp4 expression is maintained in the controls at later developmental stages (F,L) whereas the expression is down-regulated in the conditional mutants at later developmental stages (I,O, arrow heads). Bmp7 expression is initiated and maintained in both the control embryos (G,M) and conditional mutants (J,P) at ss22–24 and at ss25–27. ND, not done. Dorsal to Ventral orientation for all sections is indicated in A. Scale bar: 100 µm.