Table 3. Long-term persistence of RAM.

	Sample number	RAM at baseline	Time to Follow-up 1 (months)	RAM at Follow-up 1	Time to Follow-up 2 (months)	RAM at Follow-up 2
	TZ.09.032647	A98G	5	A98G	n.a.	n.d.
	TZ.08.017184	M41L	11	M41L	18	M41L
persistent RAM	TZ.08.017176	M41L	12	M41L	21	M41L
	TZ.08.017195	K103N	13	K103NS, Y188HY	18	K103N
	TZ.10.003309	E44D, V118I	21	E44D, V118I	n.a.	n.d.
	TZ.08.029746	D30N	12	none	n.a.	n.d.
	TZ.09.006017	V82A	18	none	n.a.	n.d.
RAM disappeared	TZ.08.022112	M184I, G190E	15	Q151QR	18	none
	TZ.09.001336	G73S	18	V179T	n.a.	n.d.
	TZ.08.023280	V75A	12	T69ST	14	T69ST
	TZ.08.017193	Y188H	6	L10V	n.a.	n.d.
	TZ.08.022112	M184I, G190E	15	Q151QR	18	none
	TZ.09.001336	G73S	18	V179T	n.a.	n.d.
new RAM	TZ.08.023280	V75A	12	T69ST	14	T69ST
	TZ.08.017193	Y188H	6	L10V	n.a.	n.d.
	TZ.08.017195	K103N	13	K103NS, Y188HY	18	K103N
	TZ.08.014495	M184I	6	no PCR product	n.a.	n.d.
	TZ.09.001323	G190E, G73S	18	no PCR product	n.a.	n.d.
unclear	TZ.09.001332	M184I, K103N	n.a.	n.d.	n.a.	n.d.
	TZ.08.017197	T215I, K103N, V82T	n.a.	n.d.	n.a.	n.d.
	TZ.10.002035	T69N	n.a.	n.d.	n.a.	n.d.

Of the 16 patients who presented with RAM at baseline, we analyzed plasma specimens collected at later time points (“follow-up 1”, “follow-up 2”) for the presence of RAM. We detected three different scenarios, including persistent RAM, disappearing RAM, and newly emerging RAM (some samples appear in more than one scenario). Time to follow-up sample is indicated in months. In some cases, no PCR product from plasma samples could be generated. The investigation for long-term stability of RAM is insofar incomplete as plasma specimens collected at later time points were not available (“n.a.”) for all patients, referred to as “n.d.” (not determined).