Figure 1. HDL stimulates glucose uptake in 3T3-L1 adipocytes.

3T3-L1 adipocytes were incubated with vehicle or stimulators for 30 min before being incubated for another 30 min with 1-[³H]-2- deoxyglucose. (A) 3T3-L1 adipocytes were incubated with vehicle (BSA), HDL (50 µg protein/ml), insulin (100 nmol/L) or indicated combination. (B) 3T3-L1 adipocytes were incubated with vehicle and HDL of indicated concentrations. (C) 3T3-L1 adipocytes were incubated with vehicle, HDL (50 µg protein/ml) at indicated time points. (D) 3T3-L1 adipocytes were incubated with vehicle, HDL (50 µg protein/ml), apoAI (50 µg/ml) and indicated combination with LY294002 (2 µmol/L), L-NAME (2 mmol/L) and PD98059 (1 µmol/L). Data are expressed as means±SEM of three independent experiments, n = 6. * p<0.05, ** p<0.01, vs vehicle; ## p<0.01, vs HDL.