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. Author manuscript; available in PMC: 2012 Sep 9.
Published in final edited form as: J Mol Biol. 2011 Jul 12;412(1):43–54. doi: 10.1016/j.jmb.2011.07.004

Figure 2. Behavior of Neu peptides in SDS-PAGE.

Figure 2

(A) The migration of Neu/V664E was examined as a function of peptide concentration from 30 to 960 ng of total peptide, or 1 µM to 32 µM, in each lane in the 10µl of loading solution applied to the gel initially. Molecular weight standards are shown on the left and right. (B) Three samples were examined in this experiment: (i) a pool of library peptides, (ii) purified Neu/WT peptide and (iii) purified Neu/V664E peptide. All peptides were labeled on a C-terminal cysteine with Alexafluor-488. Each lane contains 500 ng of total peptide in 10 µl of loading buffer (17 µM). On the left and right are molecular weight standards with the position of the 3000, 6000 and 17,000 Da standards highlighted.