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. Author manuscript; available in PMC: 2012 Sep 9.
Published in final edited form as: J Mol Biol. 2011 Jul 12;412(1):43–54. doi: 10.1016/j.jmb.2011.07.004

Figure 3. SDS-PAGE high throughput screen.

Figure 3

Example gels from the SDS-PAGE based high throughput screen. A: As described in the text, a 12x5 section of a 96-well plate contains one library member per well in SDS gel loading buffer. In the sixth row, a portion of each well above is added to give a mixture of five peptides. B: Sixty peptides are analyzed on a single gel in 12 lanes, with 5 peptides per lane. About 1 µg of Neu/V664E is analyzed in the 8th lane. Most library members migrate as monomers. C: When a putative dimer is identified (e.g. lane 11 in panel B), a second gel is run with the remainder of the five single peptide solutions to identify the dimeric peptide. In this secondary gel, a sample of Neu/V664E is included in lane 3. D: Most gels analyzed contained no dimers, as shown by this typical example.