Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Cell Biochem. 2011 Sep;112(9):2594–2605. doi: 10.1002/jcb.23186

Figure 5.

Figure 5

Stimulation of osteoblast with lipopolysaccharide (LPS; 0.1 µg/ml), C3a (1 µg/ml), C5a (0.1 µg/ml), IL-1β (0.1 ng/ml) or combinations of IL-1β/C3a and IL-1β/C5a for 24 h. The controls received medium without supplements. (A) IL-6 and IL-8 concentration in the supernatants of osteoblasts measured by ELISA (enzyme-linked immunosorbent assay); (B) IL-6 mRNA expression in stimulated osteoblasts relative to the untreated controls. Experiments were performed independently 3–7 times in triplicate or quadruplicate cultures; $ p<0.05