Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Hepatol. 2011 Jan 13;55(3):626–635. doi: 10.1016/j.jhep.2010.12.022

Figure 5. Cell cycle kinetics after knockdown of LGMN.

Figure 5

(A) Representative FACS cell cycle profiles of non-transfected (NT) and siRNA-transfected SkHep cells 72 hr after treatment with scrambled or LGMN siRNA. (B) In cells in which LGMN was silenced, there was a reduction in the fraction of cells in G2/M phase (7.4±10.9% in LGMN siRNA versus 25.6±3.5% in non-transfected; mean+SD; p<0.05), without a significant increase in the fraction of cells in G1 or S phase. Cell cycle profiles were not changed in cells transfected with scrambled siRNA. Data are mean of 3 independent experiments. (C) Knockdown of LGMN does not induce apoptosis, as measured by caspase-3 activity. Staurosporine (500 nM) was used to induce apoptosis as a positive control for caspase-3, and a caspase-3 inhibitor was used as a negative control. Bar graph shows that caspase-3 activity was not increased in response to knockdown of LGMN (p>0.05, by one-way ANOVA). Results are representative of four independent experiments (*p<0.001).